Hyphenated techniques for the characterization of seaweed bioactive compounds

The hyphenated method was created by combining a separation method with an online spectroscopy detection method. The separated mixture components from the chromatographic method will next pass through the interphase and into the spectroscopic method. The purpose of the coupling is to provide a detection that is more information-rich than that produced by a single analytical procedure for both identification and quantification. Over the past two decades, hyphenated analytical techniques have seen tremendous advancements that have greatly expanded their applicability in the study of elemental species, biomaterials, explosives, trace elements, natural products, etc. while demonstrating specificity and sensitivity. The characterization of seaweed bioactive compounds using hyphenated techniques such as GCMS, CE-MS, LC-MS, LC-FTIR, and LC-NMR is covered in detail in this article.