Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2024
  • Volume: 17
  • Issue: 1

Multi-epitopes vaccine design against Klebsiella pneumoniae based on outer membrane protein using immunoinformatics approaches

  • Author:
  • Indira Prakoso1, Alfero Putra Iryanto2, Tiara Rahayu3, Anzillina Rahma4, Muhammad Nur Aziz Ar Rizqi5, Viol Dhea Kharisma6,7, Arif Nur Muhammad Ansori8,9, Maksim Rebezov10,11,12, Pavel Burkov13, Marina Derkho13, Belyakova Natalia14, Rybakova Anna15, Vikash Jakhmola9, Rahadian Zainul16,17,*
  • Total Page Count: 8
  • Page Number: 11 to 18

1Department of Food Science and Biotechnology, Brawijaya University, Malang, Indonesia

10Department of Scientific Research, V. M. Gorbatov Federal Research Center for Food Systems, Moscow, RussiaRussian Federation

11Faculty of Biotechnology and Food Engineering, Ural State Agrarian University, Yekaterinburg, RussiaRussian Federation

12Department of Scientific Research, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, RussiaRussian Federation

13Institute of Veterinary Medicine, South Ural State Agrarian University, Troitsk, RussiaRussian Federation

14Moscow University of Technology and Management Named After K.G. Razumovsky, Moscow, RussiaRussian Federation

15Moscow Institute of Physics and Technology, Moscow, RussiaRussian Federation

16Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia

17Center for Advanced Material Processing, Artificial Intelligence, and Biophysic Informatics (CAMP-BIOTICS), Universitas Negeri Padang, Padang, Indonesia

2Department of Biotechnology, Esa Unggul University, Jakarta, Barat, Indonesia

3Faculty of Military Mathematics and Natural Sciences, The Republic of Indonesia Defense University, Bogor, Indonesia

4Department of Electrical Engineering, University of Indonesia, Depok, Indonesia

5Department of Biology, Diponegoro University, Semarang, Indonesia

6Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia

7Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, Indonesia

8Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia

9Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India

*Corresponding Author E-mail: rahadianzmsiphd@fmipa.unp.ac.id

Online published on 31 May, 2024.

Abstract

Klebsiella pneumoniae is a gram-negative of bacteria that are known to cause a variety of nosocomial respiratory tract infections including pneumonia. K. pneumoniae is also included in the ESKAPE bacteria group which has high resistance to antibiotics. Therefore, alternative treatment for K. pneumoniae infection is needed, one of which is by developing a vaccine. The aim of this study was to design a vaccine against K. pneumoniae by targeting the outer membrane protein using immunoinformatics approaches. 1,708 protein of K. pneumoniae was then screened using signalP, pred-TMBB2, and Blastp to select outer membrane proteins. The selected protein, PA1_KLEPN and BAMA_KLEP7 were then predicted using T-and B-cell Epitope Prediction on IEDB to obtain epitope regions. Vaccine design of K. pneumoniae consists of 1 BCL epitope, 2 CTL epitopes, 1 HTL epitope, an adjuvant and PADRE sequences constructed with linkers using Benchling. This vaccine construction is predicted to be non-toxic/allergenic and have a strong binding affinity with human TLR-4 with the HADDOCK score of -93.2kcal/mol, RMSD 0.5 and Z-score -2.5. According to the computer-aided studies conducted for this study, the chosen epitopes may provide excellent vaccine candidates to stop K. pneumoniae infections in people. However, in order to further confirm the efficacy of this suggested vaccine candidate, in vitro and in vivo validation is required.

Keywords

Bioinformatic, Immunoinformatics, In silico, Klebsiella pneumoniae, Multi-epitope vaccine