Isolation of new asiatic acid derivative from Centella asiatica (L.) urban as antioxidant

Centella asiatica (L.) Urban is known to have a pharmacological effect as an antioxidant. Indonesian research on gotu kola, especially the ethyl acetate fraction it produces, is rarely carried out. Previous research stated that kaempferol and quercetin which isolated from the ethyl acetate fraction of Centella asiatica herb had antioxidant activity with respective IC₅₀ values of 16.50 and 21.61 μg/mL, so this study aimed to get other compounds from the ethyl acetate fraction which has antioxidant activity. The purposes of this study were to isolate, identify, and examine the free radical scavenging activity of DPPH from the ethyl acetate fraction of gotu kola (C. asiatica (L.) Urban) herb. Isolation of the ethyl acetate fraction was carried out from 70% ethanol extract of Centella asiatica plant using vacuum liquid chromatography and gravity column chromatography afforded a new asiatic acid derivative. Elucidation of a new compound based on UV-Vis, FT-IR, LC-MS/MS, 1D-NMR and 2D-NMR to get 11-hydroxy-9-(hydroxymethyl)-1,2,6a,6b,9,12a-hexamethyl-10-((3,4,5-trihydroxytetrahydrofuran-2-yl)oxy )-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,12b,13,14b-icosahydropicene-4a-carboxylic acid and it had moderate antioxidant activity with IC₅₀ of 106.99 ± 1.76 μg/mL. The pure isolate obtained is suspected to be a new compound derived from asiatic acid.