1Angarsiddha Shikshan Prasarak Manadal’s College of Pharmacy, Sangulwadi, Sindhudurg, 416810, (MS) India
2Department of Quality Assurance, Ashokrao Mane College of Pharmacy, Peth-Vadgaon, Kolhapur, 416001, (MS) India
3Department of Pharmaceutics, Yashwantrao Bhonsale College of Pharmacy, Sawantwadi, 416510, (MS) India
4Angarsiddha Shikshan Prasarak Manadal’s College of D. Pharmacy, Sangulwadi, Sindhudurg, 416810, (MS) India
*Corresponding Author E-mail: mayuresh.redkar253@gmail.com
Online published on 31 May, 2024.
A simple, precise, accurate, sensitive cheap and selective RP-HPLC method has been developed of salmeterol in bulk and pharmaceutical formulation. The method was carried out on an Inertsil ODS C18 (250 × 4.6mm, packed with 5μ). The maximum absorbance was found to be at 203nm. The Salmeterol xinafoate followed linearity in the concentration range 20 to 70ppm and correlation coefficient was found to be 0.999. Injection volume was selected to be 20μl and the flow rate was fixed at 1.0ml/min. Mobile phase selected with the ratio of methanol and water (60:40 v/v). Run time was selected to be 8min. Retention team was found to be 3.521. The LOD and LOQ were found to be 0.00471 and 0.01429.
Salmeterol Xinafoate, RP-HPLC, UV Spectroscopy, Method development, Validation
