1Department of Pharmacognosy, Pravara Rural College of Pharmacy, Loni, (413736), Maharashtra, India
2Department of Financial Management, Sir Vishveshwaraiya Institute of Technology, Sinnar, Maharashtra, India
*Corresponding Author E-mail: sunainavikhe@gmail.com
Online published on 6 May, 2025.
Pharmacological Evaluation on effect of sour orange and Corn silk in combination on urolithiasis Ethylene Glycol induced rats.
The present study was carried out to investigate the traditional use of Corn silk and Sour Orange easy removal of kidney stones due to its diuretic activity and action in rats.
The Reflux extraction method was used to obtain the aqueous extract, then column chromatography and TLC were used for fractionation. The fraction isolated are 2-Propanone, 1-hydroxy-,2-Furanmethanol, 1,3-Butadiene-1-carboxylic acid, has been confirmed by GC-HRMS results.
The complex process of kidney stone formation, also known as urolithiasis, is brought on by a series of physicochemical processes that take place inside the kidneys, such as supersaturation, nucleation, growth, aggregation, and retention. In this present study antiurolithic activity of corn silk, sour orange, combined dose sour orange and corn silk, fraction extract investigated in animal models of urolithiasis. It is induced in rats by administering 0.75 % ethylene glycol in drinking water for 28 days and calcium oxalate crystals were confirmed by urine analysis and X rays. Then, urine analysis and X rays were done for crystals observations.
Animals were sacrificed at the conclusion of the study, and the organs were examined for histological alterations. Additionally, the biochemical factors such as calcium, oxalate, uric acid, protein, citrate, and magnesium that contribute to kidney stone formation were examined. To verify the drug's effectiveness and safety, tests on enzymes including lactate dehydrogenase and oxalate were also conducted. Significant information from the histopathology examinations and X-rays confirmed the effectiveness of corn silk and sour orange in urolithiasis. The results indicate that fraction extract of corn silk and sour orange shows significant antiurolithiatic activity.
Corn silk and sour orange has shown very good efficacy and safety, hence safe for use in humans.
Urolithiasis, Ethylene Glycol, Calcium Oxalate, Corn, Sour orange
