1Department of Food Industry Quality Insurance, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor, 16154, Indonesia
2Department of Industrial Waste Treatment, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor, 16154, Indonesia
3Department of Chemistry Analysis, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor, 16154, Indonesia
*Corresponding Author E-mail: septilinamelati.aka@gmail.com
Online published on 6 May, 2025.
The food and beverage industry often uses synthetic dyes to restore the color of food products that fade during the production process. One natural dye that can replace synthetic dyes is derived from carotenoid compounds. Apart from giving food a yellow, orange or red color, carotenoids also have an antioxidant function that can protect the body from free radicals. The many functions of carotenoids for health mean that carotenoids can also be applied to products in the form of carotenoid powder. Carotenoid pigments can be taken from the source, namely by extracting them. There are several extraction methods ranging from conventional to modern methods. Using the enzymatic carotenoid extraction method has advantages compared to other methods, namely that it does not use a lot of solvent, obtains a high extraction yield, and is environmentally friendly due to low energy consumption. Carrotenoid extraction tool can be operated to extract carotenoids from carrots with a capacity of 2.5 liters of carrot pulp. Enzyme activity test showed that the hydrolysis time was 135 minutes, pH 5, temperature 50°C and the addition of enzyme pectinase 2% and cellulase 2% was the optimum treatment with the highest reducing sugar content of 37.60mg/mL. The highest levels of carotenoids were obtained from maceration extraction with a methyl acetate solvent of 10.49%, extraction using a device with a methyl acetate solvent of 25.73%, and extraction with a tool and the enzyme methyl acetate solvent was obtained 62.00%.
Extraction, Carotenoids, Enzymes, Design, Development