Detection of presence of bioactive metabolites in CCFE (Co cultured Cell Free Extracts) of Microbacterium barkeri (LMA4), Corynebacterium argentoratense (LMA5) and Streptomyces shenzhenensis (LMA6)

The members of actinomycetes, versatile microspecies with dynamic source of bioactive molecules, are the nodal attraction of researchers. Continuous efforts are streaming to detect and launch new or derivetatised antibiotics to defend against the reemerged drug resistant infectious agents. This communication is dealt with detection of bioactive molecules from three strains of novel actinomycetal strains, namely, Microbacterium barkeri (LMA4), Corynebacterium argentoratense (LMA5) (Gene bank No. OP023130) and Streptomyces shenzhenensis (LMA6) (Gene bank No. OQ092768) isolated from pond soil of near by locality. The strains were cocultured with laboratory maintained bacteria, Staphylococcus aureus (BMS4) and a Gram negative, Escherichia coli (BME4) and the co cultured cell free extract (CCFE) of respective actinobacterial strains were subjected to UV-visible and LC MS analysis. The preliminary observation could note about similar peak pattern with Streptomycin, Doxorubicin, Pyrazine, Pyrrolizidines, Oxacillin, Ciprofloxacin, Allistatin, Gentamycin, Chlorellin, Penicillin, Penicillin G, Kanamycin, Levofloxacin, Amikacin, Ofloxacin, Imipenem and more over Ampicillin, as inferred from UV visible, followed by Liquid chromatography. The conclusive result was inferred from the LC-MS (m/z) spectrum analysis. It was noted that the peak with ID-53, eluted from the CFE, of co-culture broth of LMA4 with BMS4, with retention time (RT) 26.99 (min), of m/z 751.3, carried out with Electrospray Ionisation (ES) in +ve mode (ES+), Peaks with IDs, 45, 54 (24.65 and 27.11respective RTs), having resultant m/z, 749.8and 751.7 in ES-, and the CFE containing LMA6 and BMS4, with eluted peak Ids 12, 42 (RTs, 5.71, 20.55), having m/z 752.4 and 749.8 and 749.8 in ES+ mode, which could be assigned with structure of Azithromycin.