Qualitative and Quantitative Phytochemical Evaluation, Antioxidant and Antimicrobial activity of Hardwickia binata Roxbs. leaf extract in various solvent

Hardwickia binata Roxbs. has historically been used by indigenous cultures for the therapeutic management of various medical conditions. The leaves of the plant were successively extracted with ethylacetate, chloroform, acetone, and ethanol by soxhlet extraction. We assessed the ethyl acetate, chloroform, acetone, and ethanolic extracts for their antioxidant activity utilizing in vitro models, namely the DPPH radical scavenging assay, nitric acid scavenging, reducing power assay method, and the antibacterial properties of Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae were examined using the agar disc diffusion technique. The ethanolic extract was found to possess excellent antioxidant and antimicrobial activity as compared to other solvent extract. A preliminary analysis of phytochemicals was conducted, leading to the identification of several phytoconstituents within the extract as flavonoids, phenolic compounds, tannins, glycosides, and proteins. The antioxidant activity may be attributed to flavonoids and phenolics present in the drug.