UV Spectrophotometeric Analytical Method Development and Validation for Aloe-Emodin Phytochemical in Bulk and Formulations

Aloe- emodin is an anthraquinone derivative mainly obtained from Aloe vera plant. It is reported to have several pharmacological activities including antiviral, antiproliferative, analgesic, antidiarrheal etc. Several formulations are available in market in combination with other phytoconstituents. Hence, there is a need for an analytical method for regular analysis of aloe-emodin in marketed formulations and bulk drugs. The goal of the current study was to use a UV 1800 Shimadzu double beam spectrophotometer to design and validate a straightforward, quick, accurate, and selective analytical method for estimating aloe-emodin in bulk and in marketed formulations. Using methanol as the solvent, the spectrophotometer-based detection process was carried out at λmax of 428.5 nm. The linearity, range, specificity, accuracy/recovery, precision, and robustness of the suggested approach were all confirmed. With a correlation coefficient of more than 0.999, the aloe-emodin detector response was found to be linear over the chosen concentration range (0.1-0.7μg/mL). The accuracy was between 98.78 to 100.38%, variance of standard curve line was found to be 0.052. Among the five sample preparations, the precision (R.S.D.) ranged from 0.129 to 0.325%. The limits of quantification (LOQ) and detection (LOD) were reported to be 26.518μg/mL and 0.278μg/mL, respectively. The recovery of aloe-emodin was about 98.78 to 100.38%. The data collected showed that the commercial formulations’ excipients did not interfere with the procedure. As a result, it is convenient to use these formulations for routine quality control analyses of aloe-emodin in bulk medication, marketed capsule, and other formulations.