Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2024
  • Volume: 17
  • Issue: 11

Effectiveness of Therapeutic Hypothermia Using HBN-1 Compared to Forced Hypotermia in Neurobehaviour Improvement after Cardiac Arrest: An Experimental Animal Model Study

  • Author:
  • Prananda Surya Airlangga1, Rahmat Sayyid Zharfan1, Nancy Margarita Rehatta1,*, Soetjipto2, Eddy Rahardjo1, Widjiati3
  • Total Page Count: 10
  • Page Number: 5632 to 5641

1Department of Anesthesiology and Reanimation, Faculty of Medicine, Universitas Airlangga – Dr. Soetomo General Hospital, Surabaya, Indonesia, 60132

2Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia, 60132

3Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia, 60115

*Corresponding Author E-mail: nancy-m-r@fk.unair.ac.id

Online Published on 06 May, 2025.

Abstract

Post-cardiac arrest resuscitation commonly leaving neurological defects. Hypothermia is known to affect several physiological aspects of the brain. HBN-1 was developed as an alternative in regulated hypothermia.

To analyze the impact of HBN-1 as regulated hypothermia compared to forced hypothermia on neurobehavior after cardiac arrest in animal models.

A true experimental study, with a controlled group post-test design. On male Wistar-strain rats,after anesthesized, cardiac arrest was carried out using electric current. After ROSC, the rats were randomly divided into normothermia, force hypothermia (ice pack), and regulated hypothermia (HBN-1) group. Therapeutic hypothermia was carried out for 3 hours. The treatment group was returned to the cage and received standard rat food and drink.Neurobehavior was measured by the Rat ND Score on day-7.

The force hypothermia achieved hypothermia was significantly faster than HBN-1 (p=0.01).The hypothermic group showed significantly lower Rat-ND Score than normothermia (p=0.012). The HBN-1 group show lower Rat-ND Score than forced hypothermia, but not statistically significant (p=0.083). There was significant linear correlation between Rat-ND Score and the neuronal cell apoptosis in claustrum (p=0.000), with correlation coefficient of 0.843. The linear graphic analysis showed R2=0.6575.

Hypothermia using HBN-1 show significant decrease in Rat-ND Score. Further research in experimental animals with a larger number of samples and replication needs to be done. The variable that also needs to consider is the safety of using this pharmacological agent.

Keywords

Biomedical, Cardiac arrest, HBN-1, Hypothermia, Medical, Neurobehaviour