Research Journal of Pharmacy and Technology

SCOPUS
  • Year: 2024
  • Volume: 17
  • Issue: 2

Investigations of biological activities and characterisation of phytochemical constituents of Pulicaria laciniata extracts, an endemic plant growing in south of Algeria

  • Author:
  • Kamilia Bireche1,2,*, Hocine Dendougui1, Mine Gultekin-Ozguven2, Sara Hasni3, Yousef Rahmani4, Asma Abid1, Mahdi Belgidoum1, Mohamed Hadjadj1, Beraat Ozçelik2,5
  • Total Page Count: 7
  • Page Number: 769 to 775

1Kasdi Merbah University Ouargla, Faculty of Mathematics and Material Sciences, Department of Chemistry, Valorization and Promotion of Saharan Resources (VPRS) Laboratory, 30000, Ouargla, Algeria

2Istanbul Technical University, Faculty of Chemical and Metallurgical Engineering, Department of Food Engineering, Maslak, TR-34469, Istanbul, Turkey

3Kasdi Merbah University Ouargla, Natural Sciences and Life Sciences faculty Biological Sciences Department Lab of Protecting Ecosystems in Arid and Semi-Arid Areas., Ouargla, 30000, Algeria

4Center for Scientific and Technical Research in Physico-Chemical Analysis (CRAPC), Ouargla, Algeria

5Bioactive Research & Innovation Food Manufac. Indust. Trade Ltd., Katar Street, Teknokent ARI-3, B110, Sariyer, 34467, Istanbul, Turkey

Abstract

Since limited research was conducted on studying the Pulicaria laciniata (P. laciniata (Coss and Kral)) aerial parts, this study was carried out. This study aimed to determine and classify three main extracts constituents by GC/MS, HPLC-PDA and screen there in vitro biological potency as the Antioxidant capacity, anti-diabetic, anti-Alzheimer's and anti-obesity activities. In which three extraction was affected with Chloroform, Ethyl acetate and n-Butanol each extract was characterised with GC/MS and HPLC-PDA, after that the capacities of the extracted molecules was evaluated against the ABTS free radical, ferric iron, α-amylase, α-glucosidase, acetylcholine esterase and the pancreatic lipase in order to investigate the mentioned activities. As a result, the n-Butanol and Ethyl acetate extracts had more polyphenols than the Chloroform extract. The most potent anti-obesity and anti- anti-alzeihmer action were demonstrated by the Chloroform extract through inhibition of pancreatic lipase and acetylcholinesterase.

Keywords

Pulicaria laciniata, Antioxidant activity, Biological activities, HPLC-PDA, GC-MS