Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2024
  • Volume: 17
  • Issue: 2

Phenolic content and antioxidant activity of hydroethanolic and aqueous extracts of the inflorescences of Allium sphaerocephalon L.

  • Author:
  • Chahrazed Kaoudoune1,2,3,*, Fatima Benchikh2, Cherif Abdennour3, Hassiba Benabdallah2, Chawki Ben Souici5, Ismahane Derafa4, Walid Mamache4, Smain Amira2
  • Total Page Count: 7
  • Page Number: 903 to 909

1Laboratory of Environmental Biomonitoring, Faculty of Sciences, Department of Biology, Badji Mokhtar University, 23000 - Annaba, Algeria

2Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University of Setif 1, 19000, Algeria

3Laborat/ory of Animal Ecophysiology, Faculty of Sciences, Department of Biology, Badji Mokhtar University, 23000 - Annaba, Algeria

4Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biochemistry, Faculty of Nature and Life Sciences, University of Setif 1, 19000, Algeria

5Centre de Recherche en Biotechnologie (C.R.B.T) Constantine, Constantine, Algeria

*Corresponding Author E-mail: chahrazedkaoudoune@gmail.com, chahrazed.kaoudoune@univ-annaba.org

Online published on 1 June, 2024.

Abstract

The objective of this effort is to quantify the amounts of phenolics and the in vitro antioxidant capacities of Allium sphaerocephalon L. flowers’ hydroethanolic (EOH) and aqueous (AQE) extracts. The two extracts’ total phenolic (TPC) and flavonoid content (TFC) were determined spectrophotometrically. Results showed the extraction yield obtained by EOH and AQE were 18.30% and 11.80%, respectively. The EOH gave higher amounts of TPC (12.73±0.91μg GAE/mg DW) and TFC (7.67±0.24μg QE/mg DW) than AQE (TPC = 9.71±0.53μg GAE/mg DW and TFC= 5.68±0.90μg QE/ mg DW). Five techniques were used to train antioxidant activity. The EOH extract had high activity in DPPH radical scavenging capacities and β-carotene bleaching assay, while the AQE extract had high activity in metal chelating ions and reducing power assay. However, both extracts had no different significance in cupric-reducing capacity. A moderate quantity of flavonoids and polyphenols, as well as a satisfactory level of antioxidant activity, were observed in both preparations derived from the flowers of A. sphaerocephalon L., where this activity is not all fully related to the amount of phenolic compounds.

Keywords

Allium sphaerocephalon L, Extract, Antioxidant, Flavonoid, Polyphenol