1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia
10Department of Biology, Faculty of Mathematics and Science, University of Padjadjaran, Bandung, Indonesia
11Center for Bioprospecting of Natural Fibre and Bioresources, University of Padjadjaran, Bandung, Indonesia
12Department of Biomedicine, School of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia
13Department of Pharmacy, Faculty of Public Health, Universitas Anwar Medika, Sidoarjo, Indonesia
14Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia
15Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar, Indonesia
2Center for Advanced Material Processing, Artificial Intelligence, and Biophysic Informatics (CAMP-BIOTICS), Universitas Negeri Padang, Padang, Indonesia
3Computational Virology Research Unit Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, Indonesia
4Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia
5Bioinformatics Department, Indonesia International Institute of Life Sciences (i3L), Jakarta Timur, Indonesia
6Postgraduate School, Universitas Airlangga, Surabaya, Indonesia
7Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India
8Department of Food and Agriculture Product Technology, Faculty of Agriculture Technology, Universitas Andalas, Padang, Indonesia
9Department of Agricultural Technology, Politeknik Pertanian Negeri Payakumbuh, Payakumbuh, Indonesia
*Corresponding Author E-mail: rahadianzmsiphd@fmipa.unp.ac.id
Online published on 3 June, 2024.
Nowadays, dengue virus (DENV) is still become a global problem, even though the virus infection issues have reached half of the population in some countries each year. DENV belongs to the enveloped virus with positive-sense single-stranded RNA (+ssRNA) genus Flavivirus and belongs to the Flaviviridae family. DENV has structural proteins which consist of the envelope protein (E), capsid (C), and membrane (M). There are four serotypes of this virus which are DENV-1, 2, 3, and 4. These four serotypes are transmitted to humans through Aedes sp. The development of this vaccine is still in progress and the challenge of this DENV vaccine candidate design is to overcome the heterotypic infection and the expansion of coverage protection to all virus serotypes. This research uses design simulation for vaccine candidates using B cell epitope in all DENV’s serotypes envelope to trigger the antibody formation through bioinformatics method that consists of protein modeling, immunogenicity, toxicity, and immune stimulation. DENV envelope protein was predicted to have polytope that can be recognized by B cells and act as an antigen, have low similarity with the composing sequence of cell surface receptors on the body, and non-toxic, and then can trigger the population increase of B cell and IgM antibody production with high avidity to neutralize four of the DENV serotypes. We recommend the B cell polytype which consists of A, C, E, and G peptides be examined by the wet-lab approach.
B-cell, Bioinformatics, DENV, Flavivirus, Polytope, Viroinformatics
