1Demonstrator, Department of Microbiology, GMCDoda, J&K, India
2Assistant Professor, Department of Microbiology, GMCDoda, J&K, India
3Consultant, Department of Medicine, Raja Hospital Nawanshahr, Punjab
4Veterinary Assistant Surgeon, Department of Sheep Husbandry, Jammu and Kashmir
*Corresponding Author E-mail: sameerazargar@gmail.com
Online published on 3 June, 2024.
A new corona virus pandemic (COVID-19) has caused 7,010,568 deaths worldwide. The three stages of the Covid-19 disease course include the asymptomatic stage, upper respiratory tract infection, and lower respiratory tract infection/ARDS.
We examined the dynamical profile of SARS CoV-2 in 100 COVID-19-infected hospitalised patients from Jammu and Kashmir's Chenab valley (Doda, Bhaderwah, and Kishtwar). Patients throat and nasal swab samples were taken at various times following the onset of symptoms. RT-PCR test with a COVID-19 nucleic acid detection was used to find SARS-CoV-2. The real-time RT-PCR assay examined two target genes: nucleocapsid protein (N) and open reading frame 1ab (ORF1ab).
To assess the SARS-CoV2 positive detection rate using real-time polymerase chain reaction (RT-PCR).
At one week after the onset of symptoms, the PCR positivity rate was 100%; it then gradually decreased until it reached 100% negative results at six weeks following the onset of symptoms. Up to 6 weeks following the start of symptoms, viral shedding took place. For senior individuals, an extended monitoring period is required.
This study discovered that individuals with extended viral nucleic acid conversion were typically older and had more co-morbid conditions, such as diabetes and hypertension.
ARDS, Lower respiratory tract infection, COVID-19, SARS-CoV-2, RT-PCR, ORF lab
