Antioxidant and anti-inflammatory activity of the terpenoidal fraction of ethanolic extract of Byttneria herbacea

The goal of this study was to identify the bioactive principle of the plant Byttneria herbacea (Malvaceae) which has a wide range of traditional therapeutic uses.Column chromatography was used to separate the ethanolic extract of aerial parts of the plant with mobile phase Chloroform: Methanol (5:5). The fraction with Rf 0.62 on TLC with mobile phase Toluene:Ethyl acetate (85:15) imparting bluish color under UV 254 nm was giving the test of Terpenoids. The same fraction was assessed for Antioxidant and Anti-inflammatory activity.Antioxidant activity was performed by measuring the decolourizing activity followed by capturing the unpaired electron from the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and compared it with the standard anti-oxidant Gallic acid. To test anti-inflammatory activity in an experimental model of acute inflammation, the carrageenan-induced in rat paw edema method was employed.The antioxidant and the anti-inflammatory activities of the Terpenoid fraction were found to be satisfactory.