1Dept. of Basic Science and Humanities, Dhanekula Institute of Engineering and Technology, Ganguru, Vijayawada - 521139, Andhra Pradesh, India
2Department of Humanities and Sciences, VNR Vignana Jyothi Institute of Engineering and Technology, Hyderabad, 500090, Telangana, India
3Dept. of Chemistry, V.R. Siddhartha Engineering College, Kanuru, Vijayawada - 520 007, Andhra Pradesh, India
4Department of Chemistry, Govt. Degree College, Siddipet - 502103, Telangana (Dt), India
5Dept. of Chemistry, University College of Sciences, Acharya Nagarjuna University, Guntur - 522510, Andhra Pradesh, India
*Corresponding Author E-mail: mannamsrao@gmail.com
Online published on 13 June, 2024.
As part of this study, a new sensitive, convenient, accurate and robust reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of azelidipine in pharmaceutical and tablet formulations was developed and validated. The three variables chosen were methanol concentration, flow rate, and pH in the mobile phase. Separation was performed using an HPLC method with a UV detector and a Chromosil C18 (250mm × 450mm). Methanol was pumped at a flow rate of 1.0mL/min and adjusted to pH 4.0 with dilute OPA (60:40% v/v) and pH 4.0 with potassium dihydrogen phosphate buffer (pH 4.0), 255nm.
RP-HPLC method, Validation, Stability-indicating method
