Effect of extraction method on antioxidant activity, anti-inflammation and cytotoxicity of Curcuma aeruginosa roxb. rhizome

Temu Hitam (Curcuma aeruginosa Roxb) is recognized as one of the plants that has the potential to be utilized in the practice of traditional medicine. The selection of the extraction method is an essential initial step in the process of chemical component purification. In this particular investigation, the extraction process was carried out utilizing a variety of techniques, including hot maceration, cold maceration, and ultrasound-assisted extraction (UAE), in order to investigate the anti-inflammatory, antioxidant, and cytotoxicity capabilities of the subject material. The percent yield from the UAE method showed the highest amount of 51.94%, and the IC₅₀ values from the cold method, hot method, and UAE using the FRAP method were 4.65, 6.22, and 5.57 ppm, respectively. The extract using the UAE method has higher cytotoxicity to Artemia salina Leach larvae. In addition, UAE showed better anti-inflammatory activity with an IC₅₀ value of 2.96 ppm.