Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2024
  • Volume: 17
  • Issue: 6

Serum SIRT1 levels and genetic variants in diabetic nephropathy: Insights from a cross-sectional study

  • Author:
  • D Pai1, S Adiga2,*, G Suresh3, U Adiga4, S Kumari5, D Chaitra6, TM Desy7
  • Total Page Count: 6
  • Page Number: 2829 to 2834

1Tutor, Dept. of Anatomy, KS Hegde Medical Academy, Mangalore, Karnataka, India

2Professor, Dept. of Pharmacology, Apollo Institute of Medical Sciences and Research, Chittoor, India

3Professor, Dept. of General Medicine, KS Hegde Medical Academy, Mangalore, Karnataka, India

4Professor, Dept. of Biochemistry, Apollo Institute of Medical Sciences and Research, Chittoor, India

5Professor, Dept. of Biochemistry, KS Hegde Medical Academy, Mangalore, Karnataka, India

6Tutor, Dept. of Anatomy, KS Hegde Medical Academy, Mangalore, Karnataka, India

7Research Scholar, Central Research Lab, KS Hegde Medical Academy, Mangalore, Karnataka, India

*Corresponding Author E-mail: adigaiscool@yahoo.com

Online published on 24 April, 2025.

Abstract

The aim of the study was to compare sirtuin 1 serum levels in non-insulin dependent diabetics and diabetic nephropathy patients, and evaluate the pattern of polymorphism of SIRT 1 gene in these patients, and find the relation between polymorphism of SIRT1 gene and sirtuin1 serum levels in diabetic nephropathy patients and those with various stages of diabetic nephropathy.

108 type-2 diabetic patients without complications as controls and 108 diabetic nephropathy patients as the case group were included in the study. SIRT 1 expression was measured by ELISA, and SIRT1 gene polymorphism was analyzed using the PCR-RFLP method.

The mean serum sirtuin 1 level were significantly lower in diabetic nephropathy patients compared to controls (p=0.000). The distribution of genotypes did not conform to Hardy-Weinberg equilibrium. The frequency of the wild-type genotype (AA) was higher in the case group, while the mutant allele (AG+GG) was more prevalent in controls. The distribution of genotypes did not conform to Hardy-Weinberg equilibrium (chi-square =7.203, p=0.027). There was no significant association observed between SIRT1 gene polymorphism and serum sirtuin 1 level in diabetic nephropathy patients(p=0.001). Additionally, no significant difference was found in serum sirtuin 1 level between different stages of diabetic nephropathy based on albuminuria testing and estimated glomerular filtration rate (eGFR)(p=0.33).

Patients with diabetic nephropathy exhibited significantly lower serum sirtuin 1 level compared to controls, suggesting a potential role of sirtuin 1 in the pathogenesis of DN. We also conclude that serum SIRT 1 expression may be used as a diagnostic marker. The results indicate a need for further research to better understand the role of SIRT1 in diabetic nephropathy and its potential as a biomarker or therapeutic target for this condition.

Keywords

Type-2 diabetes, SIRT 1, Polymorphism, Genotypes