1Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi, Karnataka, India
2KLES’s College of Pharmacy, Nipani-591237, Karnataka, India
*Corresponding Author E-mail: rupesh.k88@gmail.com
Online published on 24 April, 2025.
The present study deals with development and validation of accurate, sensitive and cost-effective RP-HPLC method for Loxoprofen sodium.
PRIMESIL C18 column (id 4.6 × 250 mm length) was used as stationary phase and a mobile phase used was mixture of methanol and 0.05% OPA buffer in ratios of 75:25 v/v. The 20μl samples were injected at a flow rate of 1 ml/min. The detector used is G-13148 (DAD) detector, with a wavelength observed of 225nm at ambient column temperature. Further, the method developed was validated as per ICH guidelines.
The method's linearity was studied at concentrations between 5 and 25μg/ml, wherein the results were found to be linear with correlation coefficient (r2) values of 0.999. The limits of detection and quantitation for developed method were observed to be 0.09175 and 0.278032 μg/ml respectively. The intraday and interday precision results were analysed and found to be within 2%. The percentage assay of the marketed product was found to be 101.85±0.090%. The percentage recovery of the drug was ranged between 97.7 1% to 101.09%. during accuracy study.
ICH guidelines, Linearity, Loxoprofen sodium, RP-HPLC, Validation
