In vitro and In silico studies on antidiabetic properties of fisetin: Focus on interactions with PEPCK and GSK3β

The prevalence of diabetes throughout the world has increased alarmingly over the recent past and the scenario will be increased in the future. There are many medications available to treat diabetes, but none have proven to be the best option for a number of reasons, including unfavourable side effects and diminishing effectiveness with continued use. Global interest and attention in plant-derived medical products for the treatment of various illnesses has increased within the past ten years. Fisetin (3, 3', 4', 7 - tetrahydroxy flavone), a major flavonoid present in strawberries, onion, persimmon exhibits various biological properties such as anticancer, antioxidant, anti-inflammatory and antidiabetic effects. Fisetin exert its antidiabetic potential by regulating the key enzymes of carbohydrate metabolism. Current study is designed in evaluating the glucose uptake potential of fisetin on L6 myotubes. We have observed that Fisetin facilitated the uptake of glucose in rat L6 myotubes. To study molecular interactions, Fisetin was subjected to docking against 3D structure of Diabetic targets PEPCK and GSK3B using Autodock 4.2 and their molecular interactions were visualized using Biovia discovery studio visualizer. Docking interactions and ADMET studies of the fisetin signified the application as a natural therapeutic agent to combat diabetes.