Isolation and molecular identification of lactic acid bacteria from Annona muricata L

Annona muricata L. or Soursop leaves, known as "god leaves," have multi-active properties. Soursop leaves are proven to contain annonaceous acetogenin compounds, namely nanomedicine, and muricapentocin, which have antibacterial effects. This study aims to identify Lactic Acid Bacteria that act as probiotics. Isolation of lactic acid bacteria using special media (MRS Agar). Lactic acid bacteria were identified through two procedures, namely morphological identification with Gram stain and catalase test and molecular identification with the 16S rRNA gene as a marker for 27F and 1429R as a primer. The method used for identification is descriptive data analysis. The results showed that the isolate had the characteristics of lactic acid bacteria, namely Gram-positive, catalase-negative, and bacilli. Based on phylogenetic analysis, these bacteria have similarities/resemblance to Lactiplantibacillus plantarum strain MGEL20154 (100%).