Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2024
  • Volume: 17
  • Issue: 7

Determination of ethanol in kombucha with Gc-Fid: A validated method for halal verification

  • Author:
  • Lilis Sulistiawaty1, Maulidiawati Khoirunisa1, Imas Solihat2,*, M. Nashih3
  • Total Page Count: 6
  • Page Number: 3415 to 3420

1Department of Chemical Analysis, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor, 16154, Indonesia

2Department of Food Nanotechnology, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor, 16154, Indonesia

3LPPOM, MUI Indonesia, Jl. Pemuda No.5 Tanah Sereal, SempurBogor, 16161, Indonesia

*Corresponding Author E-mail: imaskhairani@gmail.com

Online published on 29 April, 2025.

Abstract

Determination of ethanol content on kombucha tea refers to AOAC 2016.12 using an internal standard method and by gas chromatography and is different from inhouse method. Validation parameter that performed was linearity (R2>0,990), precision (%RSD <2/3CV Horwitz), accuracy (%recovery: 95-105%), instrument detection limit (0,0015%), quantitation detection limit (0,0086%) and method detection limit (0,0328%) and these numbers has met the acceptance requirements so that this method can be used for routine analysis in the laboratory.

Keywords

Ethanol, Kombucha, Method Validation