Analysis of Aspirin (Acetylsalicylic Acid): Development and Validation using RP-HPLC Methods - A Component of Anti-hypertensive Drugs

A precise, sensitive, and focused liquid chromatographic method has been developed to assess the quantity of aspirin in its bulk form. The chromatographic conditions involved utilizing a C-18 column (250 × 4.6 mm, 5 µm particle size) with a mobile phase consisting of water containing 0.1% orthophosphoric acid (v/v) at pH 3.0 and acetonitrile in a ratio of 45:55. The flow rate was set at 1 ml per minute, and the total run time was 20 minutes, with a detection wavelength of 237 nm. The retention time was observed to be 4.01 minutes, and the method exhibited an average recovery percentage of 99.9%. The proposed procedure adhered to the guidelines established by the ICH and is applicable for analyzing aspirin as a standalone bulk drug and in combination with other pharmaceuticals.