Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2024
  • Volume: 17
  • Issue: 8

The Viability of Osteoblasts against SHED Metabolites and EGCG for Biomaterial Osteogenesis

  • Author:
  • Yuliati1,2, Fatma Yasmin Mahdani3,4, Shafa Putri Khansa7, Dealtrini Pongsumae7, Wahidah Tsamara Putri Yastuti7, Muhammad Afif Wardana7, Meircurius Dwi Condro Surboyo4, Mohammed Aljunaid1,5,6, Huda Rashad Qaid1,6, Aqsa Sjuhada Oki2, Fitria Abbas Thalib8, Rini Devijanti Ridwan2, Indeswati Diyatri2,*
  • Total Page Count: 7
  • Page Number: 4015 to 4021

1Doctoral Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132 – Indonesia

2Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132 – Indonesia

3Oral Medicine Study Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132 – Indonesia

4Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132 – Indonesia

5Department of Oral and Dental Medicine, Faculty of Medicine, Taiz University, Taiz, Yemen

6Faculty of Oral and Dental Medicine, Al-Saeed University, Taiz, Yemen

7Bachelor of Dental Science Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132 – Indonesia

8Department of Technology Pharmacy, Academy Pharmacy Surabaya, Surabaya – Indonesia

*Corresponding Author E-mail: indeswati-d@fkg.unair.ac.id

Online Published on 30 April, 2025.

Abstract

EGCG, the principal flavonoid found in green tea, exhibits numerous advantageous properties, notably promoting bone regeneration by enhancing the activity of osteoblasts and osteogenic differentiation. Cell-free therapy is an alternative to avoiding the side effects of cell-based therapy. By harnessing the potential of metabolites, SHED combined with EGCG can be a biomaterial to increase osteogenesis.

This study aims to assess the viability of osteoblast cells when exposed to the combination of SHED metabolites and two concentrations of EGCG, namely 10μM and 50μM.

Osteoblast viability is examined with the 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide (MTT) assays using an ELISA reader 570nm, and the absorbance value is converted to per cent form. CD50 is a parameter that indicates non-toxicity when the percentage value of living cells is more than 50%.

The percentage of living cells exceeded 50%, and statistically significant distinctions were observed among the control media, control cell groups, and the groups exposed to the combination of SHED metabolites and EGCG (p = 0.031).

The viability of osteoblast cells exposed to the combination of SHED metabolites and EGCG 10µM, as well as the combination of SHED metabolites and EGCG 50µM, showed no toxicity. The combination of 10µM SHED metabolites and EGCG showed a higher osteoblast cell viability value than the combination of SHED metabolites and EGCG 50 µM.

Keywords

EGCG, SHED, Osteogenesis, Cell viability, MTT, Medicine