Degradation of the industrial dyes using purified polyphenol oxidase isolated from peel and pulp of tomato

In India, Tomato is an essential horticulture crops which is an abundant source of polyphenol oxidase. Plants able to resist infections against microbial and viral due to presence of Polyphenol oxidases. Polyphenol oxidase was extracted from tomato peel and pulp with sodium phosphate buffer and purified through salt precipitation and dialysis. The enzyme activity of Polyphenol oxidase was determined by spectrophotometric assay using catechol. The specific activity of tomato peel crude enzyme was 16.296U/mg and purified enzyme was 240 U/mg whereas tomato pulp crude enzyme had the specific activity 5U/mg and 70U/mg in purified. Protein concentration of purified peel and pulp polyphenol oxidase was analysed 0.18μg/ml and 0.21μg/ml respectively. Dye degradation was also performed. Whereas auromine degraded by purified peel 80.76% and purified pulp 73.08%.