1Department of Microbiology, Haribhai V. Desai College, Pune, 411002 Affiliated to Savitribai Phule Pune University, Pune (India)
2Department of Environmental Science, Fergusson College (Autonomous), Pune, 411004 Affiliated to Savitribai Phule Pune University, Pune (India)
*Corresponding Author E-mail: ashish.mane@fergusson.edu
Online published on 30 April, 2025.
The D-limonene was extracted from orange peel and used for testing of Antimicrobial Activity, Antioxidant Activity and Most Probable Number. FTIR shows presence of chemical groups such as C-C, CH3; CH2, C=C which indicates presence of D-limonene oil in the orange peel extract. Test organism isolated from water sample, was E. coli and other test organisms namely Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Aspergillus niger were used to check the antimicrobial activity. Zone of inhibition was observed on the plates against E. coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans but no zone of inhibition was observed against Aspergillus niger. The test for antioxidant activity indicates that the D-limonene has antioxidant activity (63.69 %). The antimicrobial test was performed. The MPN test was performed before and after addition of D-limonene to water sample. The total number of coliforms were reduced after treatment of drinking with D-limonene. Therefore, D-limonene is a good alternative for the purification of drinking water.
D-limonene, Antimicrobial Activity, Antioxidant Activity, Most Probable Number
