Antioxidant, anti-inflammatory and fourier transform infrared (FTIR) analysis of flavonoid fractions from Gymnopsoria montana

Gymnosporia montana, a medicinal plant species, has been traditionally used by people for the treatment and cure of various infectious ailments. In this study, phytochemical profiling and biological evaluation (anti-inflammatory and antioxidant) of hydroethanolic leaves and fruit fractions (acetone, chloroform, and petroleum ether) obtained through the soxhlet extraction approach of Gymnosporia montana were investigated. Further, hydroethanolic fractions were also subjected to FT-IR analysis in order to influence the companionship of functional groups in miscellaneous phytoconstituents. The results of antioxidant based assays of methanolic leaf and fruit fractions showed that the acetone and chloroform fractions of leaves had the strongest DPPH antioxidant potential, which may directly have correlated with enhancement in phenolic and flavonoid content as compared to fruit fractions. However, the acetone and chloroform fractions of the leaves also showed anti-inflammatory activity against typhoid vaccine. Further, functional groups were identified using FT-IR and the results of these FT-IR studies showed the presence of various functional groups like amines, aromatic compounds, alcohols, phenols, etc., which aid in each of their medicinal properties. It is possible to conclude that the presence of different pharmacological compounds gives them different characteristics, and the presence of different functional groups aids their medicinal properties. In short, these results support the possible use of the acetone and chloroform leaf fraction of hydroethanolic leaf extract containing flavonoids from Gymnosporia montana as antioxidant and anti-inflammatory agents.