Investigation of antioxidant activity, total phenolics, and TLC profiling of Jatropha multifida petiole extract

A Jatropha multifida is a medicinal plant that is commonly used as a wound treatment medicine. In accordance to wound healing, the body’s defense involves reducing oxidative reactions using antioxidant compounds to destroy pathogenic microorganisms. One part of betadine plant is the petiole. However, no study has been reported on the antioxidant activity of the petiole. In this study, TLC (thin layer chromatograph) and DPPH (1,1-diphenyl-phicryl-hydrazyl) reagent were used to study its antioxidant activity. Phytochemical evaluation of the methanol extracts of Jatropha multifida petioles showed the presence of alkaloids, flavonoids, sterols, and phenols. The IC50 value of the antioxidant test of the extract was 569.69 ppm. Meanwhile, TLC-DPPH test suggested that the active components of the extract was phenols and the total concentration was 25 mg GAE/g extract.