Validated UV spectroscopic method for determination of domperidone in bulk and pharmaceutical formulation UV spectroscopic method

The aim of this research paper was to develop a simple, sensitive, rapid, accurate and economical Ultra Violet spectrophotometric method for the estimation of Domperidone. Various analytical parameters such as linearity, range, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were determined according International Conference on Harmonization (ICH) guidelines. Absorbance maximum (λmax) of drug in pH 6.8 phosphate buffer was found to be 233nm. Beer's law was obeyed over the concentration range. With a correlation value (r2) of 0.998, the drug followed linearity in the concentration range of 2-20g/ml, indicating strong connection between the response factor and the concentration of standard solutions. Because the %RSD values for intra-day and inter-day were observed to be less than 2%, it was determined that the created approach was accurate. At each additional concentration, good recoveries (97.11% to 101.31%) of the medication were obtained, proving the method's accuracy.The method's sensitivity was determined to be 0.0542916 and 0.045386 g/ml, respectively, for the limits of detection and quantification. The results of the study demonstrated that the developed method is accurate, precise and reproducible while being simple, cheap and less time consuming and hence can be suitably applied for the analysis of Domperidone in pharmaceutical preparations for dissolution studies.