Research Journal of Pharmacy and Technology

During surgery, the risk of complications is high. If it is not immediately controlled and treated properly, it can result in serious complications in the form of bleeding, requiring medication to treat this condition. Side effects due to hemostasis drugs can be minimized using natural ingredients. One natural ingredient that has the potential to be a hemostatic drug is edamame seeds. Edamame seeds contain chemical compounds such as flavonoids, tannins and saponins which can shorten bleeding time. The aim of this study was to analyze the effectiveness of edamame seeds (Glycine max l. Merril) extract on fibrinolytic activity test in strain BALB/c mice, including bleeding time, clotting time, and platelet count tests. Before the test was carried out, a phytochemical screening test was carried out on the active ingredients suspected to be present. Type of laboratory experimental research with the research design of the posttest only control group design. 20 mice were adapted for one week, divided into 3 groups. The negative control group (C-) was sondage Na-CMC 0.5%, the positive control group (C+) was sondage tranexamic acid dose 0.065mg/gr BW mice, the treatment group was sondage edamame seed extraction and divided into 3 subgroup doses of 0.112mg/gr BW mice (ESE1), 0.14mg/gr BW mice (ESE2), 0.168mg/gr BW mice (ESE3) in Na-CMC 0.5%. Mice were fed for 7hours before sondage administration, after 3 days of administration. All treatment groups were performed bleeding time, clotting time, and platelet count tests. The test results obtained showed that all tests conducted had the same tendency. The results of the Tail bleeding time and clotting time tests in the treatment group showed that the higher the dose, the smaller the test result value. Where the smallest value was in the ESE3 group. While the platelet count results in the treatment group showed that the higher the dose, the higher the test result value. Where the highest value was in the ESE3 group. The edamame seed extract (Glycine max L. Merril) is effective in shortening tail bleeding time, clotting time and increase platelet count in BALB/c strain mice.