Development of a UV Spectrophotometric Method and Validation of Paroxetine Hydrochloride

For the development and validation of the Paroxetine Hydrochloride method, a simple, reliable, cost-effective, and repeatable UV Spectrophotometric method was used. With a correlation value (R2 = 0.999), paroxetine hydrochloride exhibits maximum absorbance (λmax) in methanol at 294 nm and follows Beer’s law at concentrations between 10 - 60 μg/ml. By using the standard addition approach, the proposed method’s validity was evaluated. The additional standard’s mean percentage recovery was found to be between 98% and 102%. The determined values for the limits of detection and quantification were 0.206μg/ml and 0.625μg/ml, respectively. The International Conference for Harmonisation (ICH) Q2 (R1) guidelines have been followed in the validation of the method. The suggested approach is rapid, simple to use, precise, sensitive, and cost-effective, making it suitable for the regular quantification of paroxetine in pharmaceutical formulations and bulk form.