Research Journal of Pharmacy and Technology

Protease refers to enzymes that catalyze the peptide bond hydrolysis of proteins. Proteolytic enzymes has its presence in different forms of life like microorganisms, animals and plants. Among them, protease from microorganisms occupies a safe position, as they have functional characteristics and enormous industrial applications. Microbial proteases have broad uses in different industries like food, detergent, and leather in bioremediation. In the food industry, they are used in the modification, improved quality, and storage stability of protein sources and meat tenderization. The present study screened microorganisms from household wastewater for novel protease bacteria in a casein agar plate. Among the isolates, the colony with the highest zone of proteolytic activity was selected for further analysis, it was identified as Enterobacter ludwigii by molecular characterization through BLAST. The optimization was carried out for protease enzyme for pH, carbon, organic, and inorganic nitrogen sources, and incubation time determines the maximum protease production by A1 isolate were at pH 9 and 72 hours of incubation. Among different parameters used in optimization, carbon source (Sucrose) showed maximum enzyme activity of 26.4 U. It can be used for further application studies.