1Assistant Professor, Department of Conservative Dentistry, College of Dentistry, Qassim University, Kingdom of Saudi Arabia
10Professor, Department of Pediatric Dentistry, Sharavathi Dental College, Shivamogga, India
2Department of Orthodontics and Dentofacial Orthopedics, Nitte (Deemed to be University), AB Shetty Memorial Institute of Dental Sciences (ABSMIDS), Mangalore, Karnataka, India
3Associate Professor, Department of Restorative and Prosthetic Dental Sciences, College of Dentistry, Majmaah University, Zulfi, Saudi Arabia
4Lecturer, Department of Preventive Dental Sciences, College of Dentistry and Pharmacy, Buraydah Private Colleges, Buraydah, 51418, Qassim, Kingdom of Saudi Arabia
5Associate Professor of Periodontology, Department of Periodontology and Implant Dentistry, College of Dentistry, Qassim University, Saudi Arabia
6Associate Professor, Department of Oral and Maxillofacial Surgery and Diagnostic Sciences, College of Dentistry, King Faisal University, Al Hofuf, Kingdom of Saudi Arabia
7Department of Conservative Dentistry, College of Dentistry, Qassim University, Saudi Arabia
8Assistant Consultant, Department of Restorative Dentistry, College of Dentistry, King Saud University Medical City, Riyadh, Kingdom of Saudi Arabia
9Professor & HOD, Department of Oral Medicine and Radiology, Kalinga Institute of Dental Sciences, KIIT, Deemed to be University, Bhubaneswar, Odisha, India
*Corresponding Author E-mail: bkaushik0127@gmail.com
Online published on 31 March, 2026.
Still one of the most common chronic illnesses worldwide is dental caries. Materials based on fluoride can help to prevent carious lesions. Silver diamine fluoride (SDF) has recently been demonstrated to be an effective caries arresting therapy. To assess the antibacterial efficiency of SDF, sodium fluoride varnish and Silver Nanoparticle varnish on streptococcus mutan in caries prevention. In this in vitro investigation, Streptococcus mutans monospecies biofilm was inoculated into 33 saliva-coated dentine blocks that were taken from extracted primary molar teeth with deep carious lesion. Eight times per day for six days, 10% sucrose in brain-heart infusion broth was given to the biofilms. Then dentine blocks (n=11 per group) were treated with one of the following chemical agents following the biofilm formation period as: Group A- 38 % silver diamine fluoride (SDF), Group B- 5% sodium fluoride (Na F) varnish, and Group C- Neem Silver Nanoparticle varnish (SNF). After 48 hours of incubation at 37ºC, the samples were analysed to determine how many live microorganisms were present in the biofilms and also zone of inhibition for each test materials. After being treated with SDF and the Silver Nanoparticle varnish product, Streptococcus mutans was dramatically reduced (p<0.05). Conversely, SDF indicated highest antibacterial action followed by Neem Silver Nanoparticles varnish and least with sodium fluoride varnish (p<0.05).Compared to sodium fluoride varnish, SDF exhibits greater antibacterial effect against the cariogenic Streptococcus mutans biofilm. The Neem Silver Nanoparticle varnish antibacterial activity is equally effective and can be suggested to use.
Antibacterial, Dental Caries, Neem nano particle varnish, Fluoride, Streptococcus mutans
