Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2025
  • Volume: 18
  • Issue: 11

Robusta Coffee Husk Extract as a Promising Natural Compound for The Treatment of Periodontitis: A Focus on Alveolar Bone Resorption and Formation

  • Author:
  • Nadie Fatimatuzzahro1,*, Hanna Alifia Pratiwi2, Rendra Chriestedy Prasetya1, Amandia Dewi Permana Shita1, Dwi Kartika Apriyono3, Nuzulul Hikmah1, Hafiedz Maulana1
  • Total Page Count: 7
  • Published Online: Mar 31, 2026
  • Page Number: 5472 to 5478

1Department of Dentistry, Biomedical Science, Oral Pathology and Maxillofacial, Faculty of Dentistry, Universitas Jember, Jember, 68121, Indonesia

2Undergraduate Program, Faculty of Dentistry, Jember, 68121, Universitas Jember, Indonesia

3Department of Dentistry, Forensic Odontology, Faculty of Dentistry, Jember, 68121, Universitas Jember, Indonesia

*Corresponding Author E-mail: nadie.fkg@unej.ac.id

Online published on 31 March, 2026.

Abstract

Periodontitis is an inflammatory disease of periodontal tissue. Porphyromonas gingivalis is the main periodontopathogenic bacteria by inducing macrophages and lymphocytes which can stimulate an increase in Receptor Activator of κB Ligand (RANKL) and decreased Osteoprotegerin (OPG) can activate osteoclast proliferation and cause alveolar bone damage. Robusta coffee husk contains flavonoids, tannins and caffeine which act as anti-inflammatory. This study aimed to analyze the effect of robusta coffee husk extract on the number of osteoblasts and osteoclasts in the alveolar bone of Wistar rats induced by Porphyromonas gingivalis for 21 days.

Laboratory experimental research type, 24 rats were divided into 6 random groups, the normal group (KN); Porphyromonas gingivalis induced rat group (K-); group of rats induced by Porphyromonas gingivalis and given metronidazole (K+); group of rats induced by Porphyromonas gingivalis and given 125 mg/kg BW robusta coffee husk extract (KP 125); group of rats induced by Porphyromonas gingivalis and given 250mg/kg BW robusta coffee husk extract (KP 250); group of rats induced by Porphyromonas gingivalis and given 500mg/kg BW (KP 500) robusta coffee husk extract and euthanized on the 22nd day. Tissues were processed and stained with Haematoxylin Eosin (HE). Calculations of osteoblasts and osteoclasts in the alveolar bone area of the buccal part of the lower left M1 tooth, then analyzed with one-way ANOVA statistics.

There was a significant difference between the number of osteoblasts and osteoclasts KP 125 and KP 250 with KN and K+. The mean number of osteoblasts and osteoclasts did not differ significantly between KP 500 and KN and K+.

Robusta coffee husk extract at a dose 250 mg/kg BW effective to increase the number of osteoblasts and reduce alveolar bone osteoclasts in rats induced by Porphyromonas gingivalis.

Keywords

Robusta coffee husk extract, Osteoblasts, Osteoclasts, Periodontitis