Quantification and Analytical Validation of Olanzapine by Liquid Chromatographic Method through Isocratic Separation

The quantification and analytical validation of Olanzapine was developed with a stationary phase Inertsil ODS column C18 (4.6mm×250mm, 5µm) and LC1220 Agilent HPLC instrument equipped with variable wavelength detector at 270nm and Acetonitrile: Water: Triethylamine (60:40:0.1v/v/v) was used as mobile phase passed at a flow rate 1mL / min. Elution takes place at retention time of 2.143min. Validation of method were executed as stated by ICH guidelines which illustrates linearity concentration range from 10-125µg/mL amid Correlation coefficient=0.999 with Y = 47278x+63531 regression equation obtained. LOD and LOQ for Olanzapine be 0.35 µg/ml and 1.05µg/ml correspondingly. Recovery of Olanzapine was obtained 99.47-100.01%.