Evaluation of SIRT1 as a Potential Biomarker of Aging among Middle-Aged Individuals with Altered Serum Vitamin D Status

The role of Vitamin D in promoting overall cellular health and preventing age-related diseases is well established. The Sirtuin1 gene regulates cellular processes like DNA repair, promotes longevity, and is recognized as one of the molecular hallmarks of biological aging. However, the potential link between Vitamin D and SIRT I is not well-established.

The primary objective is to compare the serum sirtuin 1 level among individuals with and without vitamin D deficiency. The secondary objective is to evaluate the association of SIRT1 gene polymorphism with vitamin D levels.

87 subjects with serum vitamin D deficiency and 87 with normal vitamin D levels were participated. Serum sirtuin 1 level was measured by enzyme-linked immunosorbent assay (ELISA). Genotyping was performed with the blood sample for Single Nucleotide Polymorphism (SNP)-rs3740051 of the SIRT1 gene by using polymerase chain reaction-restriction fragment length polymorphism (PCR‒RFLP). A comparison of variables between the two groups was performed using the Mann-Whitney U test. Spearman’s correlation test assessed the correlation between sirtuin 1 and vitamin D. The association between SIRT1 gene polymorphism and vitamin D levels was analyzed by chi-square test. Hardy- Weinberg equilibrium was calculated for the alleles.

The findings of the study showed serum Vitamin D deficiency attributed to higher SIRT1 gene polymorphism and the down expression of the SIRT1 gene. Therefore, the study concludes that Individuals with vitamin D deficiency exhibit altered SERT1 gene expression mediated accelerated biological aging.