Biochemical and Pharmacological Studies to Validate the Antiarthritic Potential of Drynaria quercifolia Rhizome

The objective is to screen fresh and concentrated juice extract of rhizome of Drynaria quercifolia (L) for its anti-arthritic property using in-vivo model.

In-vivo anti-arthritic effect was evaluated by Complete Freund’s Adjuvant (CFA) induced arthritic model. Haematological and biochemical parameters were assessed in the arthritic model with and without treatment. Assays of enzymatic and non-enzymatic antioxidants, lysosomal enzymes, and protein-bound carbohydrates were performed using a standard protocol.

A Decrease in paw volume was noticed from the 16th day, and a significant reduction in paw volume was observed after the 16th day in DQ (fresh juice and extract) and standard groups. Levels of Hemoglobin, Packed Cell Volume (HPCV) and platelets were normal in all groups. Significant (p<0.05) increase of TC (Total Count) and decrease of red blood cells were observed in arthritic group when compared with normal group. There was a significant change in liver enzyme levels. The activities of enzymatic antioxidants like catalase, dismutase and peroxidase in tissue homogenates also showed positive results. There was significant (p<0.05) increase in levels of lysosomal enzymes (alkaline phosphatase, acid phosphatase, cathepsin D) in the arthritic control animals. This was controlled by the treatment with DQR (extract and fresh juice) and standard drug (MTx). Histology reports confirm mild cartilage erosion in the DQ fresh juice-treated group.

Our findings highlighted the promising effects of fresh juice and extract of Drynaria quercifolia rhizome in managing arthritis.