Novel Stability-Indicating RP-HPLC Method for Estimation of Bexagliflozin in Bulk and Pharmaceutical Dosage form

The current technique sought to estimate Bexagliflozin by employing accurate, simple, specific, sensitive RPHPLC procedure. Ascentis C18 [150mm×5.0mm,2.8µm particle size]column and mobile phase with ratio Methanol:(pH-4) 0.01N Potassium dihydrogen ortho phosphate [40:60 v/v]was used to design the present analytical RP-HPLC method with PDA detector of maximum wavelength 225nmat 1.2ml flow rate. Bexagliflozin was eluted at 2.857min retention time. The method linearity was evaluated at 5-30µg/ml concentration. The projected limit of quantification for bexagliflozin was 0.23µg/ml and the limit of detection for it was 0.08µg/ml. At 100.14%, Bexagliflozin achieved a range of percentage recovery. The results showed that Bexagliflozin had an intra-day precision of 0.7 and an inter-day precision of 0.8 %RSD. Studies were conducted in accordance with the guidelines set forth by the ICH. Bexagliflozin was estimated usingan accurate trouble shootingRP-HPLC method.