In vitro Anti-aging properties of Seablite (Suaeda maritima) Root Extract on Skincare Application

Suaeda maritima (L.) Dumort is a grassy plant in mangroves area. The root is used for treatment of skin diseases in Thai traditional medicine. Nevertheless, there is a sparely scientific report. This study was aimed to assess the phytochemical composition, total phenolic and flavonoid contents contained in S. maritima root extract (SMRE). There anti-aging of skin of SMRE were evaluated by antioxidant properties, anti-tyrosinase and inhibition of melanogenesis, matrix metalloproteinase-2 (MMP-2) inhibition, and induction of telomerase activity. The qualitative phytochemical screening of SMRE was positive for saponins, anthraquinones, flavonoids, tannins, triterpenes, and steroids. The total phenolic content (TPC) and total flavonoid content (TFC) of SMRE were 191.3±3.55mg GAE/g and 21.2±2.09mg QE/g, respectively. SMRE was scavenged DPPH radicals (SC₅₀ =0.29±0.02mg/ml) and inhibited lipid peroxidation (IPC₅₀ = 0.08±0.03mg/ml). SMRE (1.0mg/ml) was toxic against human skin fibroblasts, while it was lack of cytotoxicity on B16F10 melanoma cells and HeLa cells for all concentration (0.0001-1.0mg/ml). MMP-2 of human skin fibroblasts was inhibited 7.24±2.17% and 14.04±3.55% after treated with SMRE and vitamin C at 0.1mg/ml, respectively. The telomerase activity of HeLa cells after treated with SMRE (1.0mg/ml) was increased 1.35±0.24% when compared with untreated cells. SMRE was slightly inhibited mushroom tyrosinase (IC₅₀ = 356.35±50.4mg/ml). Therefore, the tyrosinase and melanin production of B16F10 melanoma cells after treated with SMRE (1.0mg/ml) were inhibited 41.26±8.75% and 22.43±6.00%, and the tyrosinase and melanin production after treated with kojic acid (1.0mg/ml) were 86.63±8.29% and 52.43±5.49%, respectively.