Determination of Total Flavonoids and BSLT Methanol Extract of Leaves and STEM Betadine (Jatropha multifida Linn.)

Betadine plant (Jatropha muktifidia Linn.) is traditionally used as a medicine that can heal wounds and pain due to accidents slashes. However, research on the total flavonoids and toxicity in plant was rarely done. To analyze the total flavonoids toxicity in the extract of betadine, betadine’s leaves and stems were extracted using methanol. The phytochemical assay revealed that all extracts contain Alkaloids, Flavonoids, Saponins, Sterol, and Phenol. The total flavonoids of Betadine leave extract were higher than Betadine stem extract. Betadine leaves extract has 10,36±0,03mg E Q/g and otherwise, Betadine stem extract has 1,13±0,02mg E Q/g of total flavonoids. A toxicity test was being to Artemia saline with BSLT (Brine Shrime Lethality Test) methods. The result showed that Betadine leaves and stem extract has high toxicity because their LC-50 was 29,29 ppm and 27,79 ppm.