Analytical profiling of Clopidogrel Bisulphate: A Method Development and Validation by UV-Spectrophotometer using Simulated Nasal Fluid

Clopidogrel, an adenosine diphosphate inhibitor derived from Ticlopidine, has not previously been quantified in simulated nasal fluid using a straightforward UV-spectrophotometric approach, as revealed in the existing literature. In this study, we established a simplified UV-spectrophotometric technique specifically for assessing Clopidogrel Bisulphate in simulated nasal fluid. The standard curve was constructed within the concentration range of 0.5 to 3µg/ml, and the method underwent comprehensive validation for accuracy, precision, robustness, and reproducibility. The absorption maxima were identified at 242nm, and the method exhibited excellent linearity within the concentration range of 0.5 to 3µg/ml, boasting a correlation coefficient of 0.996. The developed method proved its accuracy, with the mean recovery value closely approximating 95%. Additionally, the limit of detection (LOD) and limit of quantification (LOQ) were determined to be 0.193 and 0.6434, respectively. Overall, this newly devised and rigorously validated method stands out for its simplicity, accuracy, precision, cost-effectiveness, and reproducibility.