Extraction and determination of quercetin and ascorbic acid simultaneously in tomatoes (Solanum lycopersicum L.) using mean centering of ratio spectra

The study aims to extract and quantify the levels of quercetin and ascorbic acid in tomatoes simultaneously without separation. This is achieved through the creation of a spectrophotometric method using mean centering of ratio spectra.The preparation of the absorption spectrum for ascorbic acid, and quercetin with methanol, and measuring mean centering of ratio spectra of ascorbic acid at a wavelength at 238nm, regression equation Y = 0,0279X – 0,0004 and quercetin at 206 nm are Y = 0,1787X+0,1402. The levels of quercetin and ascorbic acid in tomatoes are obtained from quercetin at 470mg/100g Tomato fruit or percentage was (0.47±0.03)% and ascorbic acid at 1310mg/100g or (1.31±0.007)%. The validation parameters, namely exactness, correctness, continuance, LOD, and LOQ, indicate the mean centering of ratio spectra method for determination of simultaneous quercetin and ascorbic acid in tcmato fruit meets the requirements of the ICH guidelines.