Biological activity of Curculigo latifolia extract as skin antiaging: In vitro and silico studies approach

Aging is a very complex biological-chemical process and is influenced by both internal and external factors. Several biochemical pathways that modulate premature aging include increased reactive oxygen/nitrogen species, extracellular matrix degradation, and other pathways. This study aims to evaluate the antioxidant capacity, anti-hyaluronidase, and human dermal fibroblast cell proliferation of C. latifolia extract to determine antiaging activity. Each extract was evaluated for its antioxidant capacity by various test methods, including DPPH, ABTS, NO, TAC, and ferrozine. Anti-aging activity with anti-hyaluronidase and fibroblast cell proliferation parameters was evaluated in vitro and continued in silico using target proteins 2BCJ and 3EJH.The test results showed that the ethanol and ethyl acetate extracts from roots stems, and leaves tended to have high antioxidant capacities in the DPPH and ABTS methods up to >1000 mM/g extract, followed by the NO, TAC, and ferrozine methods. Ethanol extract at a concentration of 200 mg/L gave inhibition (%) >50% while other extracts <50%. Fibroblast cell proliferation testing showed that C. latifolia root extracts, maintained cell viability up to >100% at 500 mg/L compared to other extracts. In silico evaluation of the 2BCJ and 3EJH target proteins showed that the compounds Aviprin and 3-ter-butyl-4-methoxyphenol provided a multitarget protein interaction. In contrast, mono-target interactions were described in sugiol, guaiacol, stigmas-3,6-dione, and lucialdehyde B. We assumed that C. latifolia root extract has the potential to be developed as a dermal antiaging candidate.