1Faculty of Pharmacy, Universitas Halu Oleo, Kendari, 93232, Indonesia
2Department of Pharmaceutical Sciences, Oregon State University, Corvallis, Oregon, 97331-3507, United States of America
3Thammasat University Research Unit in Drug, Health Product Development, and Application (DHP-DA), Department of Pharmaceutical Sciences, Faculty of Pharmacy
*Corresponding Author E-mail: adryanfristiohady@uho.ac.id
Online published on 30 May, 2025.
This study aims to evaluate the anti-inflammatory activity of Lycopene in mice with ear oedema and in vitro activity. In this study, mice were used and performed in vitro anti-inflammatory tests of Lycopene with the HRBC method and protein denaturation. Moreover, the anti-inflammatory assay of Lycopene was also evaluated in xylene-induced right-ear mice. Diclofenac sodium solution was used as a positive control. The results showed that Lycopene provides anti-inflammatory activity in vitro by stabilizing the human red blood cell (HRBC), preventing lysis, and inhibiting protein denaturation with IC50 32.47 μg/ml. In addition, Lycopene also reduced the thickness of xylene-induced oedema in the right ear of mice. Lycopene provides anti-inflammatory properties by inhibiting HRBC lysis and protein denaturation and stabilizing HRBC, decreased the thickness of Oedema in the right ear of mice and the TNF-α levels.
Lycopene, Anti-Inflammatory, HRBC, Protein denaturation, Oedema
