1Dental Implant Research Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
10Undergraduate Student, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
11Membership of Faculty of Dental Surgery, Royal College of Surgery, United Kingdom
12Military Dental Officer of Royal Medical and Dental Corps, Malaysian Armed Forces
13Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
2Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
3Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
4Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
5Department of Dental Material, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
6Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
7Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
8Department of Dental Public Health, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
9Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, 60132
*Corresponding Author E-mail: rini-d-r@fkg.unair.ac.id
Online published on 30 May, 2025.
Periodontopathogenic bacteria are Gram-negative anaerobic bacteria that often cause peri-implantitis such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum.
This study is to analyze the periodontopathogen antibacterial properties of Polymethylmethacrylate (PMMA) and Hydroxyapatite (HA) composites against the growth of F. nucleatum, P. gingivalis, and A. Actinomycetemcomitans in vitro.
Inhibition zone of PMMA-HA against periodontopathogenic bacteria was carried out with the well diffusion method. Doxycycline 100 mg was used as a positive control while the treatment group were consisted of 5 groups; 1) the PMMA group, 2) PMMA-HA from balai besar keramik (BBK), 3) PMMA-HA group of nano particle size, 4) the HA-BBK group and 5) the HA-nano group. All samples from the treatment and positive control groups were placed on agar plate that has been inoculated with bacteria are incubated for 24 hours at temperature of 37°C and were measured the inhibition zone diameter by using the digital caliper. The data was analysed using analysis of variance (ANOVA) and the post-hoc Tukey Honest Significant Different (HSD) test (p<0.05).
PMMA-HA has the ability to inhibit the growth of F. nucleatum, P. gingivalis, A. Actinomycetemcomitans in vitro. The antibacterial activity of PMMA-HA nanocomposite against F. nucleatum, P. gingivalis, and A. Actinomycetemcomitans was higher than PMMA-HA-BBK (p=0.0001; p<0.05).
PMMA-HA nanocomposite has the ability to inhibit the growth of F. nucleatum, P. gingivalis, and A. Actinomycetemcomitans better than PMMA-HA-BBK which can be considered as a candidate for dental implant biomaterials with periodontopathogenic antibacterial property to prevent peri-implantitis.
Polymethylmethacrylate, Hydroxyapatite, Dental Implant, Dentistry, Medicine
