Standardization of developed Kumaryasava formulation utilizing physicochemical, spectral and chromatographic techniques

In recent years the worldwide necessity of alternative medicinal system has led to progress of traditional system of medicines. The modern scientific techniques and in depth knowledge is the today’s need.Kumariasava is one of the popular formulations constituting Aloe vera as key ingredient. The standardisation of Ayurvedic medicine assures the quality, safety and efficacy of herbal product. The study aimed to standardize developed Kumariasava formulation for chromatographic (TLC, HPTLC, HPLC) and spectral studies including UV, IR, NMR and fluorescence spectroscopy. The extracts of formulation were evaluated in various solvents amongst which chloroform extract has recorded three well-resolved fluorescent components. However, fractions (III-V) obtained amongst these extract recognised as an important parameter for standardization of KS. The spectral and chromatographic analysis of fraction V confirmed the presence of anthraquinones that is key component of aloin. Hence, aloin thus preferably utilized as a marker component for standardisation of KS. The results recorded for KS extracts were compared with the standard aloin marker compound. The overall study revealed that the developed KS formulation exhibits standards as per pharmacopeia specifications.