A Rapid and Sensitive RP-HPLC Method for the Determination of Phytoconstituents Gallic acid, Ellagic acid and Zingerone in Siddha Polyherbal Formulation Kabasura Kudineer

The Siddha formulation Kabasura Kudineer, which is used in traditional medicine has several properties such as immunomodulatory, antiviral, anti-pyretic, anti-inflammatory and hepatoprotective. In the present research a new novel approach was established and validated to identify phytochemicals ellagic acid, gallic acid and zingerone present in Kabasura Kudineer herbal formulation by RP-HPLC method. However very few methods have been reported for the concurrent quantification of these phytoconstituents. A C₁₈ Column (250 mm × 4.6 mm × 5µm) was utilized for separation of phytochemicals, the mobile phase was streamed containing ACN, Methanol and 0.1 % OPA with a ratio of 20:10:70, with 1.0 mL/min flow rate, consisting maximum wavelength 254 nm, temperature was maintained at 40°C and 20 mins run time. The technique is sensitive and may be used to identify desirable phytoconstituents present in polyherbal formulations. The proposed HPLC approach has been observed to be accurate, precise, linear, rugged, robust, and convenient for the identification of the phytoconstituents. The linearity results for each phytochemical were found to be 0.9990 which was within the permissible limits. The current research is an excellent standardization technique for the concurrent quantification and detection of phytochemicals available in herbal preparations and in complementary alternative medicines.