1Department of Internal Medicine, Faculty of Medicine, Universitas Syiah Kuala and Dr. Zainal Abidin Hospital, Banda Aceh, Indonesia
2Department of Radiology, Faculty of Medicine, Universitas Syiah Kuala, Darussalam, Banda Aceh, Indonesia
3Department of Oral Biology, Dentistry Faculty, Universitas Syiah Kuala, Darussalam, Banda Aceh, Aceh, Indonesia
4Department of Dental Material, Dentistry Faculty, Universitas Syiah Kuala, Darussalam, Banda Aceh, Aceh, Indonesia
Wound healing is a complex biological process involving various cells, extracellular matrix, and signal mediators. Chitosan, with its bioactive properties, has shown promising potential as an ingredient in wound treatment. Chitosan formulation in film form increases sustained drug release, mechanical protection in the wound area, and antioxidant properties that can protect tissue from oxidative damage. The objective of the work was to evaluate in silico and in vivo the cytotoxic response of chitosan films (CF) on oral mucosa wound healing. The film of chitosan has good antioxidants (6.09 ppm) and contains chemical elements oxygen (69.4%), calcium (19.7%), magnesium (6.6%), and phosphorus (4.3%). The chitosan compounds have a strong binding affinity for receptors (genes) involved in wound healing and remodeling injured tissue and bone (VEGFR2, IL-1β, MMP-9, CTGF, and MT-3). Both miLogP and TSPA have been identified as bioactivity indicator values potentially acceptable for wound application. The chitosan film has antioxidant qualities, chemical elements, and good bioactivity value.Varying concentrations (10%, 20%, and 30%) of chitosan film have a good effect in preventing toxicity to mucosal epithelial cells. The chemical compound chitosan film has a strong binding affinity with genes involved in wound healing and has a good effect in preventing toxicity to mucosal epithelial cells.
Chitosan, Antioxidant, Bioactivity score, Molecular docking, Wound healing gene, Mucosal toxicity
