GC-MS Characterization and in vitro Anti-Inflammatory Activity of Fruit Extracts of Flacourtia indica (Burm.F.) Merr

Plants have been used extensively as medicine for centuries to treat a variety of ailments. The current work aimed to screen for the existence of bioactive ingredients using GC-MS and to test the anti-inflammatory effectiveness of ethanolic and aqueous fruit extracts of the Flacourtia indica plant. The GC-MS analysis revealed a total of 24 Phytoconstituents in the ethanolic extracts and 6 compounds in the aqueous extracts of the Flacourtia indica plant. The results of the GC-MS analysis indicated that the bioactive components found in the fruit extracts are biologically active. Among the compounds identified N-Hexadecanoic acid was the most abundant anti-inflammatory agent (18.032%) present in the ethanolic extract of Flacourtia indica fruit. Hence the invitro anti-inflammatory activity of fruit extracts were determined by protein denaturation and HRBC membrane stabilization methods. The anti-inflammatory test yielded promising findings, with high levels of inhibition by ethanolic extracts, including 64.23% in HRBC membrane stabilization and 70% in protein denaturation procedures.