1School of Pharmacy, Suresh Gyan Vihar University, Mahal Jagatpura, Jaipur, Rajasthan, India, 302017
2Department of Pharmaceutical Chemistry, Dr. J.J. Magdum Trust’s, Dr. J.J. Magdum Pharmacy College, Jaysingpur, Dist - Kolhapur, Maharashtra, India, 416101
3Department of Pharmacognosy, Teerthankar Education Society’s, Dr. Shivajirao Kadam College of Pharmacy, Kasabe Digraj, Taluka - Miraj, Dist - Sangli, Maharashtra, India, 416305
*Corresponding Author E-mail: reachpallu58@gmail.com
Online Published on 29 July, 2025.
Researchers looked at the HepG2 liver cancer cell line to see whether ethanol and an aqueous extract of Cissuswoodrowii leaves and stems had any anticancer effects.
Using the soxhlet equipment, ethanol and water-based plant extracts were produced for this study. Using the MTT assay, trypan blue, and brine shrimp lethality assay, in-vitro anticancer action of certain plant extracts was investigated at doses ranged from 10 to 100µg/ml against designated cell line.
The anticancer action was achieved with the usage of 5-flurouracil. In comparison to the water-based stem and leaf extracts of Cissuswoodrowii Santapau, ethanolic stem extract had the greatest % of inhibition against the HepG2 cell line, along with effective IC50 values.
In conclusion, our results suggest that the 50% ethanol-aqueous stem and leaves extract of Cissuswoodrowii (Stapf Ex T. Cooke) Santapu showed a clear cytotoxic and brine shrimp lethality inductive impact on the human hepatoma (HepG2) cell line, suggesting an anticancer activity.
Cytotoxicity, MTT assay, Hep G2 cell lines, Brine shrimp lethality assay, Cissuswoodrowii
